General Cell Based Assays
Montana Molecular Green Cilia-targeted cADDis cAMP Assay - 10mL
Measure real-time cAMP signaling kinetics in the primary cilia with this live cell fluorescent assay. Add 5HT6-tagged cADDis to your cells and measure robust signals the next day on a fluorescence imaging system. Decreasing green fluorescence indicates ciliary cAMP increase. This assay is genetically encoded and packaged in BacMam for efficient transduction in most cell types - including uniform and near 100% efficiency in HEK293 cells as well as expression in many primary or iPSC-derived cells. The kit includes the cilia-targeted cAMP sensor BacMam and 500mM sodium butyrate in H2O.
Eurofins Discoverx PathHunter eXpress mRXFP4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0929E2MCP2M)
The PathHunter mRXFP4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. insulin-like 5, relaxin-3) based activation of mRXFP4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mRXFP4 and EA-tagged β-Arrestin-2. Activation of mRXFP4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM CK Beta 81/CCL23 ASY
The Human CK-beta-8-1/CCL23 DuoSet ELISA is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CK-beta-8-1/CCL23 in cell culture supernatants serum plasma and other biological fluids This immunoassay contains recombinant human CK-beta-8-1/CCL23 and antibodies raised against the recombinant factor allowing for the detection of natural and recombinant human CK-beta-8-1/CCL23 The ELISA has a sensitivity of 7 8 pg/mL and a detection range of 15 6-1000 pg/mL This product can be used to measure CK-beta-8-1/CCL23 levels for the study of chemokine biology and inflammation
Meso Scale Discovery U-PLEX Mouse MIP-1 ASY
The Meso Scale Discovery U-PLEX Mouse MIP-1alpha Assay Kit is a multiplex immunoassay that measures mouse macrophage inflammatory protein 1-alpha (MIP-1alpha) in serum plasma and cell culture supernatants The assay uses electrochemiluminescence (ECL) detection technology and provides high sensitivity up to 5 logs of dynamic range and low sample volume requirements This assay kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with an ECL tag calibrators and diluent The assay takes approximately 3 5 hours to run and 10 L of sample is required The kit can test up to 80 samples in duplicate
Meso Scale Discovery U-PLEX Rat Leptin ASY
The Meso Scale Discovery U-PLEX Rat Leptin Assay Kit is a multiplex immunoassay that measures leptin levels in rat serum plasma and cell culture supernatants The assay uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and intra- and inter-assay precision This kit includes a 96-well plate coated with capture antibodies detection antibodies labeled with ruthenium calibrators and controls The assay requires a small sample volume and provides results in as little as 3 5 hours With minimal hands-on time this kit allows for the rapid accurate quantification of rat leptin
Meso Scale Discovery R-PLEX Human IL-23R ASY
The Meso Scale Discovery R-PLEX Human IL-23R Assay is a multiplex immunoassay for measuring IL-23R in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection on patterned arrays for high sensitivity and a wide dynamic range The kit includes a plate with capture antibodies detection antibodies labeled with an electrochemiluminescent compound and calibrator and control samples for standard curve generation and assay validation It quantifies IL-23R with high precision and reproducibility suitable for 96 tests on MSD platforms
Eurofins Discoverx InCELL Hunter eXpress A549 GLP Methyltransferase Assay, 10-plate Kit. (96-0045E15CP7L)
The InCELL Hunter eXpress A549 GLP Methyltransferase Assay provides an easy-to-use cell based assay to study small molecule drug candidates that engage with regulators of epigenetics pathways. This kit assesses compound-engagement-based stabilization of GLP via detection of GLP levels. This product uses EFC technology. The cell line included overexpresses GLP target protein fused to ProLabel. In the absence of compound that binds and stabilizes the target, this protein is degraded and no EFC signal is produced. When compound engages and stabilizes the target, EA contained in the detection reagent produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress PTGER3 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0640E2CP0M)
The PathHunter PTGER3 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. prostaglandin E2) based activation of PTGER3 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PTGER3 and EA-tagged β-Arrestin-2. Activation of PTGER3 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Sphingomyelin Colorimetric As
Sphingomyelin is an important lipid component of cell membranes and lipoproteins. Cayman’s SM Assay provides a specific, sensitive, and convenient method for quantifying SM in plasma or serum in a 96-well format with a colorimetric readout at 595 nm.
Eurofins Discoverx PathHunter Human GPR83 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR83 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR83 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human GPR143 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human GPR143 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure GPR143 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter Human TACR3 β-Arrestin Cell-Based Assay Kit (CHO-K1)
The PathHunter Human TACR3 β-Arrestin Stable Cell Line Assay (CHO-K1) contains a stable clonal cell line used to measure TACR3 (GPCR) activity via recruitment of β-Arrestin2. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter AGTRL1 Bioassay Kit, 2-Plate. (93-0250Y2-00121)
The PathHunter AGTRL1 Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. apelin-13) based activation of AGTRL1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged AGTRL1 and EA-tagged β-Arrestin-2. Activation of AGTRL1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress EGFR/ErbB2 Dimerization Assay, 10pl Kit. (93-1051E3CP14L)
The PathHunter EGFR/ErbB2 Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. EGF) induced dimerization of EGFR/ErbB2 receptors. This product uses EFC technology. The cell line included overexpresses ErbB2 target protein fused to PK alongside EGFR target protein fused to EA. Upon ligand-induced dimerization of EGFR/ErbB2, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.